Nitric Oxide Alters the Pattern of Auxin Maxima and PIN-FORMED1 During Shoot Development

Hormone patterns tailor cell fate decisions during plant organ formation. Among them, auxins and cytokinins are critical phytohormones early development. Nitric oxide (NO) modulates root architecture by the control of auxin spatial patterns. However, NO involvement coordination shoot organogenesis remains unclear. Here, we explore effect development using a phenotypic, cellular, genetic analysis in Arabidopsis thaliana get new insights into characterization NO-mediated leaf-related phenotypes. homeostasis mutants impaired several architectural parameters, including phyllotactic patterns, inflorescence stem elongation, silique production, leaf number, margin. Auxin distribution is key feature for tissue differentiation need to be controlled at different levels (i.e., synthesis, transport, degradation mechanisms). The phenotypes resulting from introduction cue1 mutation axr1 resistant pin1 backgrounds exacerbate relationship between auxins. Using reporter DR5:GUS , observed an increase maxima under NO-deficient mutant scavenging, pointing NO-ASSOCIATED 1 (NOA1) as main player related production this process. Furthermore, polar transport mainly regulated PIN-FORMED (PIN1), which controls flow along margin venations. Analysis PIN1 protein shows that its accumulation development, impacting mediated mechanism building. With these findings, also provide evidence opposite effects determine architecture, terms overproduction.